Elisa enzymelinked immunosorbent assay is a platebased assay technique designed for detecting and quantifying peptides, proteins, antibodies and hormones. Faqs for pge2 elisa kit nbp229336 0 there are no specific faqs related to this product. Wash buffer prepare the wash buffer by diluting 5 ml of the supplied concentrate with 95 ml of deionized water. Originally described by engvall and perlmann 1971, the method. Pge2 prostaglandin e2 elisa kit lsf32354 is a 96well enzymelinked immunosorbent assay for the quantitative detection of mouse pge2 prostaglandin e2 in plasma, serum, tissue homogenates. This can be stored at room temperature until the kit expiration date, or. Pge2 was measured using a competitive elisa kit from cayman chemical co. Ellsworth rd ann arbor, mi usa prostaglandin e 2 elisa kit monoclonal item no. Depending on the protein of interest, antibodies, buffers, or substrates being used, this general protocol. This package insert must be read in its entirety before. The elisa is based on the competitive binding enzyme immunoassay technique. Detection of other special sample please contact our technical support. Rat prostaglandin e2 pge2 elisa kit this product is suitable for in vitro quantitative detection of rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant pge2 concentration. Ehpge2 product description a competitive immunoassay for the quantitative determination of prostaglandin e.
Reconstitute the 100 dtn pge2 tracer with 6 ml eia buffer. This elisa kit used for quantitative determination of pge2 in serum, plasma and other biological. It is a sandwich assay which can detect pge2 prostaglandin e2 down to 2. Pge2 has a number of biological actions, including vasodilation, both anti and proinflammatory action, modulation of sleepwake cycles, and facilitation of human immunodeficiency. The specificity of the pge2 elisa was investigated using authentic pge2 and fatty acids which, based on their structure, might be anticipated to compete with pge2 for binding to antibodies against pge2. General information 3 table of contents general information 3 materials supplied. Kge004b contains sufficient materials to run an elisa on one 96 well plate. Pge2 elisa kit colorimetric ka0326 these standard curves are provided for demonstration only. Duoset elisa kits can be used to develop a sandwich immunoassay and are an economical alternative to buying separate antibodies and protein standards. The mouse il2 elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of mouse il2 in serum, plasma and cell. It is recommended that the urine sample be diluted 4fold with 1x sample dilution buffer and 100 ul of sample added directly to the elisa plate well. Catalog number kge004b catalog number skge004b catalog number pkge004b for the quantitative determination of prostaglandin e 2 pge 2 in cell culture supernates, serum. Prostaglandin e2 pge2 is formed in a variety of cells from prostaglandin h2, which is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. Rebag any unused strips and frames, and store at 2 to 8c for future use.
Prostaglandin e elisa kit monoclonal cayman chemical. Mouse il2 elisa kit for serum, plasma and cell culture. Hamster prostaglandin e2 pge2 elisa kit catalog number. Pge2 prostaglandin e2 elisa kit lsf27108 is a 96well enzymelinked immunosorbent assay for the quantitative detection of human pge2 prostaglandin e2 in plasma, serum. A competitive inhibition reaction takes place between the the precoated pge2 and the pge2. This protocol works well for most samples, but may need optimization for sufficient purification of pge2 from all samples.
The detectx prostaglandin e2 pge2 multiformat elisa kit is designed to quantitatively measure pge2 present in serum, plasma, urine, tissues, saliva and culture media samples. The human il2 elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of human il2 in serum, plasma, cell culture. Pge2 elisa for the quantitative determination of prostaglandin e2 in cell culture, saliva, serum, urine and whole blood. Add prepared labeled apconjugate to appropriate wells.
Pge2 elisa kit standard curve pge2 elisa kit lot to lot performance. Canine prostaglandin e2, pge2 elisa kit 96 tests operating instructions for research use only. Prostaglandin e2 elisa kit okeh02544 assay methodology. Supernatants from the mlr were collected after 7 days of culture and evaluated for il17 production by elisa. Jan 05, 2017 in support of cox2 upregulation, these cells also exhibited an increase in endogenous pge2 production as shown with elisa in the cell lysate fig. Oxidation of arachidonic acid by prostaglandin synthases cox1 and cox2 produces prostaglandin h2 pgh2, which is further metabolized by pge synthases into its major product, pge2. Pge2 induced in and released by dying cells functions as an. Pge2 promotes breast cancerassociated lymphangiogenesis by. The microtiter plate provided in this kit has been precoated with an antibody specific to prostaglandin e2, during the. Elisa kit for prostaglandin e2 pge2, competition elisa. This elisa kit is used for in vitro quantitative determination of pge2. Originally described by engvall and perlmann 1971, the method enables analysis of protein samples immobilized in microplate wells using specific antibodies.
The elisa genie rat prostaglandin e2 pge2 elisa kits are designed for the competitive measurement of analytes in a wide variety of samples. The assay will recognize both natural and recombinant human. The density of color is proportional to the amount of pge2. Porcine prostaglandin e2 pge2 elisa kit this product is suitable for in vitro quantitative detection of porcine serum, plasma or cell culture supernatant and organizations in the natural and recombinant pge2 concentration. A competitive inhibition reaction takes place between the the precoated pge2 and the pge2 in the sample with the biotinlabelled antibody. It is a competitive eia assay which can detect pge2.
As explained on page 11 of the protocol booklet, in order to help preserve the pge2, we. Split samples into two equal parts and place each sample into clean test tubes. Elisa technical guide and protocols thermo fisher scientific. The micro elisa plate provided in this kit has been precoated with pge2. In support of cox2 upregulation, these cells also exhibited an increase in endogenous pge2 production as shown with elisa in the cell lysate fig. Like most eicosanoids, it does not exist preformed in any cellular reservoir.
Assay protocol 20 preparation of assayspecific reagents 22 plate set up. View all protocols, troubleshooting, illustrated assays and webinars. This assay is designed to detect and quantify the level of human prostaglandin e2 in biological samples. Tissuecell lysate have not been internaly validated so we do not have specific protocols. Mouse pge2 prostaglandin e2 elisa kit competitive eia. This competitive elisa kit is for determination of pge2 prostaglandin e2 levels in biological samples. The enzyme linked immunosorbent assay elisa is a powerful method for detecting and quantifying a specific protein in a complex mixture. Overview abcam s prostaglandin e2 pge2 in vitro competitive elisa enzymelinked immunosorbent assay kit is designed for the accurate. Prostaglandin e2 pge2 elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Use within 4 weeks of reconstitution and store 4 c. Standards, test samples, and biotinconjugated reagent are added to the wells and incubated.
Its activity influences inflammation, fertility and parturition, gastric mucosal integrity, and immune modulation. This elisa kit uses the competitive elisa principle. Human pge2 prostaglandin e2 quant elisa kit sandwich lsbio. Pge2 multiformat elisa kit arbor assays arbor assays. Parameter pge2 assay catalog number kge004 for the quantitative determination of prostaglandin e2 pge2 in cell culture supernates, serum, plasma, and urine. It is a competitive eia assay which can detect pge2 prostaglandin e2 down to 31. Overview abcam s prostaglandin e2 pge2 in vitro competitive elisa enzymelinked immunosorbent assay kit is designed for the accurate quantitative measurement of prostaglandin e2 in serum, saliva, urine and tissue culture media and other biological fluids.
Elisa basics guide 5 elisa technology elisa technology elisa formats the first step in an elisa experiment is the immobilization of the antigen in a sample to the wall of the wells of a microtiter plate. Please read the complete kit insert before performing this assay. Human il2 elisa kit for serum, plasma, cell culture. Whether youre looking for sandwich, direct, indirect, competitive, inhibition, bridging, capture or another elisa protocol. Tissuecell lysate have not been internaly validated so we do not have specific protocols for them however a number of users have had successful results using these sample types. Protocol summary prepare all reagents, samples, and standards as instructed add standards and samples to appropriate wells. The pge 2 eia kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Cea538ge, elisa kit for prostaglandin e2 pge2, panspecies general, competition elisa, pg e2, designed by cloudclone corp. Elisa procedure determine the number of 8well strips required for the assay. The sandwich elisa protocol provided here is representative of most readytouse elisa kits for measurement of cytokines, chemokines, growth factors, and other extracellular targets. Incubate 2 hours at room temperature or 4 c overnight. Association between the levels of prostaglandin e2 in.
Biovisions pge2 elisa kit is a competitive elisa assay for the quantitative measurement of pge2 in serum, plasma and cell culture supernatants. Prostaglandin e2 pge2 is produced in multiple cell types from pgh2, a primary product of the arachidonic acid metabolism, via the prostaglandin synthase. Allow kit components to come to room temperature for at least 30 minutes before use. Targetspecific elisa kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. The kit uses a monoclonal antibody to pge2 to bind, in a competitive manner, the pge2 in the sample or an alkaline phosphatase molecule which has pge2 covalently attached to it. C the excess reagents are washed away and substrate is added. Dilute the antigen to 12 ugml in coating solution 2. As todays scientists demand high quality consistent data for high impact journals, elisa genie have developed a range of sensitive, fast and reliable elisa. Prostaglandin e2 pge2 elisa kit e4637 biovision, inc. Below are two protocols for measuring pge2 in plasma or serum using starting volumes of 1. Prostaglandin e 2 pge 2 is a primary product of arachidonic acid metabolism in many cells. Pge2 conjugate allow the conjugate to warm to room temperature. Synonyms prostaglandin e2,dinoprostone, search name canine prostaglandin e2 elisa kit, prostaglandin e2 elisa kit, dinoprostone elisa kit intended use.
Pge2 is the main product of two cyclooxygenases, cox1 and cox2, of which cox1 is constitutively expressed and cox2 is induced by various stimuli such as cytokines and various pamps 12. This pge2 elisa kit is a competitive assay that can be used for quantification of pge2 and has a range from 7. I would appreciate any suggestion on elisa kits to measure pge2 from mouse tissue and if you can comment on sample preparation or you have a protocol that worked for you. A standard curve should be generated for each set of samples assayed. Any unused conjugate should be aliquoted and refrozen at or below 20 c. Porcine prostaglandin e2 pge2 elisa kit this product is suitable for in vitro quantitative detection of porcine serum, plasma or cell culture supernatant and organizations in the natural and recombinant pge2. Screen inhibitors of cox ii activity by measuring the levels of downstream pge 2. This elisa kit is used for in vitro quantitative determination of pge2 in samples. Elisa kit is designed to quantitatively measure pge 2 present in serum, plasma, urine, saliva, cells, tissue, and tissue culture media samples.
Prostaglandin e2 monoclonal ab solution preparation. Please refer to references 1518 for details of extraction protocols. Endogenous pge2 promotes the induction of human th17. Pge2 displays several biological functions, such as vasodilatation and smooth muscle relaxation, and is involved in pro and antiinflammation pathways. The assay is based on the competition between pge 2 in the standard or sample and alkaline phosphatase. As todays scientists demand high quality consistent data for high impact journals, elisa genie have developed a range of sensitive, fast and reliable elisa kit assays to meet and exceed those demands. Caymans pge2 elisa kit monoclonal is a competitive assay that can be used for quantification of pge2 in urine, plasma, tissue culture supernatants, and other. The elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. The prostaglandin e2 kit is designed for the rapid detection of pge2 in cell supernatant and whole cells. Perform the elisa for pge2 according to the instructions of the manufacturer.
The kit uses a monoclonal antibody to pge2 to bind, in a competitive manner, the pge2 in the sample, standard, or an alkaline phosphatase molecule which has pge2 covalently attached to it. The elisa genie mouse prostaglandin e2 pge2 elisa kits are designed for the competitive measurement of analytes in a wide variety of samples. Apr 05, 2016 pge2 is an eicosanoid lipid mediator that can exert various effects including cell proliferation, stem cell expansion, and immunosuppression. In vivo, pge 2 is rapidly converted to an inactive metabolite,14dihydro15keto. Human pge2 prostaglandin e2 quant elisa kit sandwich. Prostaglandin e2 pge2 is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Screen inhibitors of cox ii activity by measuring the levels of. Pge2 elisa kit enzo life sciences adi900001 product. Pge2 promotes breast cancerassociated lymphangiogenesis. Add 100 ul of diluted antigen to appropriate wells. A competitive immunoassay for the quantitative determination of prostaglandin e2 in biological. Depending on the protein of interest, antibodies, buffers, or substrates being used, this general protocol may need to be optimized. Csbe17168ha for the quantitative determination of endogenic hamster prostaglandin e2 pge2 concentrations in serum, plasma and. Rat prostaglandin e2 pge2 elisa kit this product is suitable for in vitro quantitative detection of rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant pge2.
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